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Objective:To investigate the characteristics of bacterial community in upper gastrointestinal tumors.Methods:The study population was patients with upper gastrointestinal tumors (esophageal cancer and gastric cancer). Gastroscopy was performed on the enrolled patients ( n=17), and the specimens were taken from the tumor sites. At the same time, non-tumor tissues more than 4 cm away from the tumor tissues were taken as the control. After total DNA was extracted and purified, high-throughput 16S DNA gene sequencing was used to detect the microbiota in tumor tissues and control tissues. Bioinformatics analysis was carried out and the differences between groups were compared. Results:16S DNA PCR showed that there was no significant difference in bacterial load between tumor tissues and control tissues. The α-diversity and β-diversity indexes showed that the community composition of the two groups was similar; the samples were discrete and the colony composition was different, but there was no significant difference between the two groups. The results of Venn diagram showed that there were more operational taxonomic units (OTUs) in non-tumor tissues than in tumor tissues (2 068 vs 1 358), indicating that the bacterial species in normal tissues were more abundant than those in tumor tissues. Compared with the control tissues, the percentages of Prevotellaceae ( Prevotella), Lactobacaceae ( Lactobacillus) and Fusobacteriaceae ( Fusobacterium) in tumor tissues were relatively higher (the average percentage was more than twice that of the control). Further paired comparison of the top ten bacteria in the family and genus abundance of the two groups of samples showed that Pseudomonas decreased significantly in tumor tissues at the family ( P=0.041) and genus ( P=0.041) levels, while Prevotella was significantly enriched in tumor tissues at the family ( P=0.031) and genus ( P=0.007) levels. Conclusions:The bacterial community in the tumor microenvironment of the upper gastrointestinal tumor changed, and the species enriched in the tumor site were mainly oral common anaerobic bacteria, such as Prevotellaceae ( Prevotella), Lactobacaceae ( Lactobacillus) and Fusobacteriaceae ( Fusobacterium), especially Prevotellaceae ( Prevotella).
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OBJECTIVE：To standardize the use of children’s over-the-counter（OTC）drugs in China，control the risk of children’s self medication and ensure the safety of children’s medication. METHODS：The questionnaire was randomly distributed to pharmacy practitioners in retail pharmacies in 13/parents in 16 provinces and municipalities directly under the central government. The results of questionnaire survey were analyzed descriptively. The corresponding suggestions were put forward for the problems existing in the use of OTC drugs for children among pharmacy practitioners in retail pharmacies and parents.RESULTS：Totally 5 367 people filled the questionnaire at pharmacies，and 5 353 questionnaires were filled validly with effective rate of 99.74％；the number of consumers（parents）who filled the questionnaires was 6 111，and 5 094 questionnaires were filled validly with effective rate of 83.36％. The results of survey showed that 93.67％ of retail pharmacies were equipped with 1-2licensed pharmacists；most of the employees in pharmacies had college degree（46.59％），and their professional knowledge was relatively weak，and only 30.00％ of them were very familiar with pharmaceutical professional knowledge. Pharmacy practitioners could provide basic pharmaceutical care，but they were not sensitive to the information of children’s age and weight；only 44.11％of the practitioners would approve the dosage according to the age and weight of the children；98.09％ of pharmacies would regularly conduct OTC drugs knowledge training，but they did not pay attention to the knowledge of children related to drug use. About half of the pharmacy practitioners were very clear about the indications，usage and dosage，precautions of OTC drugs forchildren. About 70％ of parents did not fully understand the difference between prescription drugs，green OTC drugs and red OTC drugs，and did not know the risk of home OTC treatment；63.06％ of parents chose OTC drugs according to their previous experience， and 23.05％ of parents relied on drug advertisements to choose OTC drugs； 92.64％ of parents would read the drug instruction carefully before using OTC drugs， but they had blind spots in understanding many contents of the drug instruction. In the process of OTC drug use，46.21％ of parents had used adult OTC drugs for their children，and 41.54％ of parents did not convert the amount of children；52.89％ of parents said that their children suffered from adverse reactions after taking OTC drugs. CONCLUSIONS：The pharmacy practitioners have low education on the whole，professional level needs to be improved，their understanding of children’s OTC drugs is not comprehensive enough，and there is a lack oftraining on children’s medication knowledge. Parents have a poor awareness of children’s OTC drugs，and there are many problems in the process of using children’s OTC drugs，such as inaccurate dosage，using adult’s drugs. It is suggested that we should improve the access standard of pharmacy practitioners and increase the number of licensed pharmacists；organize special training on safe drug use for children to improve the pharmaceutical service ability of pharmacies；strengthen the supervision of retail pharmacies and formulate pharmaceutical care standards for the use of OTC drugs for children；strengthen the publicity and education on rational drug use for children and popularize the knowledge of safe drug use of OTC drugs for children；give full play to the guiding role of pharmacists and pay attention to the medication guidance of parents.
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Objective:To assess the present situation of selenium nutrition in adults and school-age children in Kaschin-Beck disease area of Binxian, Shaanxi Province, and provide the basis for taking targeted measures.Methods:Binxian was divided into five grades according to the condition of Kaschin-Beck disease, and 11 representative villages were selected as the survey villages in 2018. Each survey village was located in five directions: east, west, south, north, and middle. Two household villagers were selected from each location, and adult hair samples were collected for hair selenium content determination. And school-age children hair samples were collected at the township central primary schools and 1 village primary school corresponding to the five ward levels, and the selenium content was determined.Results:A total of 85 adult hair samples were collected, the average selenium content was 237.1 μg/kg, generally at the marginal selenium deficiency level (200 - < 250 μg/kg), and there was no significant difference among different grades of disease areas (χ 2 = 5.616, P > 0.05) . A total of 162 children hair samples were collected, the average selenium content was 301.3 μg/kg, generally at the middle selenium nutrition level (250 - < 500 μg/kg), and there was significant difference among different grades of disease areas (χ 2 = 13.627, P < 0.05) . Conclusion:Although Binxian has got rid of the risk of Kaschin-Beck disease as a whole, the selenium nutrition levels of some adults and children are still low, so it is still necessary to strengthen the prevention and control measures, especially for school-age children living in a relatively serious historical disease villages.
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Objective: To explore the value of ultrasound scanning of the posterior capsule thickness of shoulder (PCT) and glenohumeral distance (GHD) for clinical staging of frozen shoulder (FS). Methods: Totally 100 patients with clinically diagnosed unilateral frozen shoulder were recruited. According to clinical staging standards, 51 shoulders were divided into stageFS group (freeing stage), 35 shoulders into stage Ⅱ FS group (frozen stage), 14 shoulders into stage III FS group (thawing stage), while 100 unaffected side shoulders of FS patients were taken as control group. Ultrasonic findings of the posterior capsule of shoulder and GHD were observed, while PCT and GHD of the affected shoulders and the unaffected shoulders were all measured and compared. Results: In stageFS group, the margin of the posterior capsule of the affected shoulders were clear, there was no significant difference of PCT between stage I FS group and control group (P>0.05), while GHD of the affected side shoulders were larger than that of the control group ([3.14±0.44]mm vs [2.22±0.35]mm, P0.05). Conclusion: Ultrasound scanning of PCT and GHD can contribute to clinical staging of frozen shoulder.
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Objective To investigate the value of multiparametric-MRI in predicting the T stage,the infil-tration,limitation and lymph node accuracy of bladder cancer. Methods A total of 55 patients underwent HR T2WI,DWI and DCE-MRI examination.The only diagnosis and the diagnosis combined diagnosis of bladder can-cer,tumor staging and infiltration were compared,and pathological diagnosis was used as the standard.ROC curve analysis was used to analyze the value of ADC in predicting the invasion of bladder cancer. Results There were 35 cases of stage T1,15 cases of stage T2,3 cases of stage T3 and 2 cases of stage T4.The accuracy of multipara-metric-MRI in staging of bladder cancer was 87.27%,which was better than that of DWI(80.00%),DCE-MRI (74.00%).HR-T2WI(K =0.768)has some advantages over DCE-MRI(k=0.545),DWI(k=0.648)and HR T2WI(k=0.280).The accuracy of multiparametric-MRI diagnosis of myasthenic invasive bladder cancer and localized bladder cancer was 87.27%,which was better than that of HR-T2WI,DWI and DCE-MRI.The AUC of ADC value was 0.989 for diagnosis of invasive bladder and 1.54 × 10-3mm2/s for diagnostic bladder cancer. The AUC of ROC curve for non-localized bladder cancer was 0.984 and the diagnostic cutoff point 1.42 × 10-3mm2/s. Conclusions The use of Multiparametric-MRI has high diagnostic performance in the diagnosis of bladder cancer in patients with T staging,myometrial invasion and tumor extent.
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Objective To explore MRI manifestations of extrapleural solitary fibrous tumor(SFT)and to analyze the reasons of misdiagnosis.Methods MRI data of 9 cases with extrapleural SFT proved by pathology were analyzed retrospectively.Lesions location, size,shape,intensity,degrees and patterns of enhancement were assessed,and the imaging features and causes of misdiagnosis were also analyzed.Results Among 9 lesions,1 was located in the right cerebellopontine angle region,2 were in subdural-extramedullary region,4 were in the abdominal cavity and 2 were in the pelvic cavity.7 cases were misdiagnosed before operation including 1 as meningioma, 2 as schwannoma,2 as mesenchymal stem cell tumor,1 as pancreatic carcinoma and 1 as Castleman disease.8 lesions were isointense and 1 was mixed iso-or slightly hyperintense on T1WI.3 lesions were isointense,4 were mixed iso-or slightly hyperintense and 2 were mixed slightly hyperintense,iso-or hypointense on T2WI.3 lesions showed significant homogeneous enhancement after the administration of contrast agent,1 lesion showed varying degrees of enhancement and the enhancement was more remarkable in isointense area on T2WI than peripheral hyperintense area.4 lesions showed heterogeneous peripherial enhancement in the early phase,progressive filling in the late phase and finally remarkable homogeneous enhancement.1 lesion showed predominant peripheral striped enhancement in the early phase,and progressive filling and mildly inhomogeneous enhancement with patchy unenhanced area in the center of the lesion in the late phase.Conclusion The MRI features of extrapleural SFT have certain characteristics.It needs to be carefully diagnosed to improve the diagnostic accuracy.
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Objective To analyze the effects of Hual qi huang granules on children with mycoplasma pneumoniae pneumonia.Methods A randomized,multicenter parallel controlled clinical trial was carried out.A total of 3 000 cases of hospitalized children with mycoplasma pneumoniae pneumonia were selected.All of them were given treatment for mycoplasma pneumoniae pneumonia with macrolide antibiotics and symptomatic treatment.They were randomly divided into 2 groups:research group and control group.The children of research group were give oral Huai qi huang granules for three months.According to the classification of pneumonia,these two groups were divided into:lobar pneumonia research group,lobar pneumonia control group,lobular pneumonia research group,lobular pneumonia control group.The hospitalization duration of fever,length of hospital stay,the absorption area of lung inflammation and pneumonia severity sores were observed.The frequency of upper respiratory infections,bronchitis,pneumonia were observed in 3 months after discharge.Results 2 378 cases were investigated.The hospitalization duration of fever,length of hospital stay of research group were significantly shorter than that of in control group (P ＜ 0.001).The children with lobar pneumonia,2 weeks after treatment,the absorption of consolidation of the lobar pneumonia research group is significantly better than lobar pneumonia control group (P ＜0.001).After two weeks treatment,the pneumonia scores of lobar pneumonia research group is lower than lobar pneumonia control group (P ＜ 0.05).Followup of 3 months after hospital discharge,frequency of upper respiratory infection and bronchitis of research group,were significantly lower than that of control.In addition,appetite increased significantly in research group than control (P ＜ 0.001).There are 21 cases with drug associated adverse reactions (mild diarrhea),including 12 cases of research group,9 cases of control group,and there was no statistical significance (P ＞0.05).Conclusion Standard treatment combined with oral Huai qi huang granules in the treatment of mycoplasma pneumoniae pneumonia,can significantly shorten hospitalization duration of fever,length of hospital stay and reduce the severity score of pneumonia.Three months oral Huai qi huang granules can significant reduce the frequency of respiratory infections and bronchitis,also can increase patients appetite,and be safe.
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Nowadays,transjugular intrahepatic portosystemic shunt (TIPS) has already become the main means for the treatment of portal hypertension and its complications due to cirrhosis.The Viatorr stent that is specifically designed for TIPS has been available in China since the end of 2015,but it has not been widely used in clinical practice in China so far.At present,superimposed release dual-stent technology by using one bare stent and one covered stent,as simulation of Viatorr stent,to perform TIPS is still employed in many clinical centers in China.Dual stent technology carries unique advantages,although the long-term efficacy of dual-stent implantation technology is not as good as that of single-stent implantation technology.This paper aims to make a comprehensive review about the dual-stent technology used for TIPS,focusing on the dual-stent technique classification and advantages,the causes and the managements of postoperative shunt dysfunction,etc.so as to let this technique can be mnore effectively used in treating portal hypertension caused by cirrhosis.
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Antibody drugs often show "heterogeneity",including the related isomers differing from one another in glycosylation,charge or molecular size.Most of these isomers come from post-translational modifications,such as aggregation,degradation,glycosylation,oxidation,deamidation or disulfide misfolding,of the recombinant protein in the "cell factories".These modifications not only influence the quality,safety and efficacy of the antibodies,but also serve as an important indication of product quality throughout the whole process of antibody production.This paper reviews the relationship between glycoslation,charge and size heterogeneities of monoclonal antibodies and drug efficacy,safety,pharmacokinetics as well as immunogenicity,contributing to a better understanding of the relationship between antibody structure and function.It will provide some support and guidance for the research and development of antibody drugs,especially biosimilars.
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This study was aimed to evaluate the clinical effect and safety of Qing-Fei Tong-Luo (QFTL) ointment for treating children with pneumonia.Randomized controlled trial (RCT) was conducted among 460 cases of children with pneumonia.The observation group was given QFTL ointment combined with basic treatment.And the control group was only treated by basic treatment.Evaluation was given on the total clinical efficacy,disappeared time of fever,cough,expectoration,shortness of breath,and medication safety.The incidence of respiratory diseases was followed up on the 30th days after drug withdrawal.The results showed that in the aspect of clinical efficacy between two groups,the cure rate of the observation group was 98.26％,and that of the control group was 93.89％,with statistic significance (P ＜ 0.05).The cure rate of the observation group was better than that of the control group.There was statistical difference on expectoration disappeared time (P ＜ 0.05).There was no statistical difference on disappeared time of fever,cough and shortness of breath (P ＞ 0.05).There was statistical difference on the incidence of respiratory diseases on the 30th days followed-up after drug withdrawal (P ＜ 0.05).There was no statistical difference on the incidence of upper respiratory tract infection,pneumonia and asthma (P ＞ 0.05).No adverse reactions occurred in the observation group.It was concluded that QFTL ointment combined with basic therapy on the treatment of pneumonia in children was significantly better than the control group in the aspect of clinical efficacy,expectoration disappeared time and the incidence of bronchitis.It is safe and effective.The prognosis is good and worthy of promotion in the clinical practice.
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Objective To observe the curative effect of acupoint injection of compound Danshen injection in the treatment of nonneoplastic epithelial disorders of vulva.Methods 96 patients with vaginal epithelial non tumor like lesions were enrolled in this study,including 38 cases of squamous epithelial hyperplasia,lichen sclerosus in 42 cases,lichen sclerosus associated with squamous cell hyperplasia in 16 cases.Take Zhōngjí,Guīlái,Huìyīn, Sānyīnjīao,Zhàohaǐ,and Tàichōng six points,each acupoint injected compound Danshen injection 2mL,injected twice a week,10 times for a course of treatment,after 2 weeks began next course of treatment,a total of three courses.The clinical curative effect and incidence of adverse reaction of three types of nonneoplastic epithelial disorders of vulva were compared.Results The effective rates (effective rate =clinical control +effective +valid)of three types of epi-thelial disorders of vulva tumor like lesions were squamous epithelial hyperplasia 92.11%,lichen sclerosus 90.48%, hardening of lichen planus associated with squamous cell hyperplasia 87.50%,there was no significant difference among the three types of lesions(χ2 =4.102,P >0.05).The total effective rate of three types of nonneqlastie epithe-lial disorders of tumor was 90.62% by acupoint injection,and there were no serious adverse reactions in all patients during therapy.A patient of lichen sclerosus appeared the symptoms of nausea,sweating during treatment due to nerv-ous,and after psychological guidance the symptoms disappeared,re -treatment did not appear.Conclusion The acu-point injection of compound Danshen injection in the treatment of vulvar intraepithelial non -tumor like lesions has better clinical effect,and has no serious adverse reactions.
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Objective To assess the effect and feasibility of group reminiscence therapy on depression in the elderly.Methods We searched the Cochrane Central Register of Controlled Trials (-2013),Medline (1982-2013),Embase (1974 2013),AMED (-2013),CINAHL(-2013),CMB (1994-2013),VIP,CNKI and Wanfang to collect the randomized controlled trials (RCTs) in which the group reminiscence therapy was used to treat depression in elderly patients.The studies were selected according to the inclusion and exclusion criteria by two reviewers,and the quality of included studies was evaluated according to Cochrane Handbook and performed meta analyses by using the Cochrane Collaboration's RevMan 5.2 software.Results A total of 10 RCTs involving 944 patients were included.The results of meta-analysis showed that as compared with conventional treatment,group reminiscence therapy on the basis of conventional treatment was more effective in decreasing depression score [MD=-4.86,95 ％ C I(-5.10,-4.65)].Compared with psychological counseling,group reminiscence therapy had more effect on decreasing depression score [MD=-9.34,95％ CI (-10.77,-7.91)].There was no significant difference in the dropout rate between group reminiscence therapy and other control treatments [RR=1.22,95％ CI (0.79-1.88)].The descriptive analysis showed that group reminiscence therapy was effective in alleviating the symptoms of depression.Conclusions Group reminiscence therapy can improve the symptoms of depression in elderly patients and conforms to the principle of economic benefit.However,the results should be interpreted with caution because of the low quality of the included studies.
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Objective:To explore the relationship between dyslipidemia and blood pressure variability (BPV)in pa-tients with essential hypertension.Methods:A total of 251 patients with essential hypertension (EH),who were treated in our department of outpatient from Jan 2011 to Dec 2012,were selected.There were 114 patients with EH complicated dyslipidemia were regarded as EH+dyslipidemia group and 137 pure EH patients reganed as pure EH group.All patients received ambulatory blood pressure monitoring (ABPM);BPV indexes were compared between two groups.Results:Compared with pure EH group,there were significant rise in standard variations (SD)of 24h systolic blood pressure (SBP)and diastolic blood pressure (DBP)[(10.59±2.04)vs.(14.96±4.12),(10.16± 2.11)vs.(12.34±3.19)],24h SBP coefficient of variation (SBPCV)[(0.13±3.07)vs.(0.91±2.21)];daytime (d)SBPSD and DBPSD [(11.27±1.97)vs.(13.98±4.03),(8.94±2.01)vs.(11.34±3.12)],dSBPCV [(0.79±0.21)比 (1.01±0.31)];nighttime (n)SBPSD and DBPSD [(7.96±3.14)vs.(13.22±5.37),(8.13±1.97) vs.(9.95±3.47)],and nSBPCV and nDBPCV [(0.78±0.18)vs.(0.09 ±0.43),(1.07±0.34)vs.(0.13 ± 0.37)]in EH+dyslipidemia group,P <0.01 all.Conclusion:There are relationship between dyslipidemia and blood pressure variability in patients with essential hypertension.
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Objective To evaluate the clinical application of preoperative multi-slice computed tomography (MSCT) and multi-slice computed tomography portography (MSCTP) in performing transjugular intrahepatic portosystemic stent shunt (TIPSS) combined with gastric coronary vein embolization (GCVE). Methods A total of 126 patients with cirrhosis complicated by upper gastrointestinal bleeding or massive ascites due to portal hypertension were enrolled in this study. The patients were arranged to receive TIPSS together with GCVE. Before the treatment, MSCT and MSCTP were performed in all patients. By using post-processing techniques, including maximum intensity projection (MIP), multiplanar reformation (MPR), volume rendering (VR) and surface shade display (SSD), the anatomy of liver was comprehensively evaluated. Results Both MSCT and MSCTP could clearly display morphologic changes of liver , the spatial relationship of the portal and hepatic veins , the degree and extent of portal collateral circulation , and the severity of ascites, which provided important anatomical information for preoperative evaluation of TIPSS and GCVE. Conclusion MSCT and MSCTP are non-invasive and reliable examinations for the diagnosis of cirrhosis with portal hypertension, it can further clarify the diagnosis and guide the performance of TIPSS and GCVE.
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Objective To evaluate the clinical application of preoperative multi-slice computed tomography (MSCT) and multi-slice computed tomography portography (MSCTP) in performing transjugular intrahepatic portosystemic stent shunt (TIPSS) combined with gastric coronary vein embolization (GCVE). Methods A total of 126 patients with cirrhosis complicated by upper gastrointestinal bleeding or massive ascites due to portal hypertension were enrolled in this study. The patients were arranged to receive TIPSS together with GCVE. Before the treatment, MSCT and MSCTP were performed in all patients. By using post-processing techniques, including maximum intensity projection (MIP), multiplanar reformation (MPR), volume rendering (VR) and surface shade display (SSD), the anatomy of liver was comprehensively evaluated. Results Both MSCT and MSCTP could clearly display morphologic changes of liver , the spatial relationship of the portal and hepatic veins , the degree and extent of portal collateral circulation , and the severity of ascites, which provided important anatomical information for preoperative evaluation of TIPSS and GCVE. Conclusion MSCT and MSCTP are non-invasive and reliable examinations for the diagnosis of cirrhosis with portal hypertension, it can further clarify the diagnosis and guide the performance of TIPSS and GCVE.
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Objective This study aims to investigate the clinical value of percutaneous vertebroplasty (PVP)combined with strontium-89 (89 Sr)on patients with spinal malignant tumor.Methods Seventy four patients who were diagnosed as spinal malig-nant tumor were divided into two groups randomly.37 patients of the control group were treated by PVP only,and 37 patients of joint group were treated by PVP and 89 Sr together.Then the clinical effect of the joint group and control group was compared after treatment.Results After treatment,the quality of life,ratio of pain relief,clinical effect and survival time in joint group were dis-tinctly better than control group (P <0.05).Conclusion PVP combined with 89 Sr can be an effective and safe treatment for pa-tients with spinal malignant tumor.
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<p><b>OBJECTIVE</b>To evaluate the effects of Embelin on HL-60 cells by the impact of oxidative stress on DNA double-strain breaks (DSBs).</p><p><b>METHODS</b>HL-60 cells were treated with Embelin in different concentration (3, 10, 30, 100, and 300 μg/ml) for 24 h, and inhibitory effects was examined by CCK-8 assay. Reactive oxygen species (ROS) levels were evaluated by flow cytometry using DCFH-DA. Comet assay was used to detect the extent of DSBs.</p><p><b>RESULTS</b>Embelin inhibited proliferation of HL-60 cells in a dose-dependent manner. At the concentration of 10, 30, 100, and 300 μg/ml, the inhibition rate was (12.74 ± 2.27)%, (23.49 ± 1.96)%, (30.30±1.89)%, and (57.55 ± 3.59)% (P<0.05). Embelin also lead to high level of intracellular ROS and deterioration of DNA damage (P<0.05). When HL-60 cells were pretreated with ROS scavenger N-acetyl-l-cysteine (NAC) for 2 h and then treated with 300 μg/ml Embelin for 24 h, the intracellular ROS level declined and DSBs relieved (P<0.05). Meanwhile, embelin-induced cell viability significantly declined to (32.75 ± 2.70)% (P<0.05).</p><p><b>CONCLUSION</b>Embelin induced the death of HL-60 cells by increasing the generation of intracellular oxidation and the oxidative stress, which drived the damage of DNA double-strand.</p>
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Humans , Acetylcysteine , Apoptosis , Benzoquinones , Cell Survival , Comet Assay , DNA Damage , Fluoresceins , HL-60 Cells , Oxidative Stress , Reactive Oxygen SpeciesABSTRACT
Objective To evaluate the embolic effect on rabbits arteries using self-made copper plated platinum coil.Methods 1 7 New Zealand Big Ear Rabbits were selected.Unilateral subclavian artery or carotid artery was embolized with self-made secondary level copper plated platinum micro-coils (experimental group)through 3F-catheter.Contralateral subclavian artery or carotid artery was also embolized using secondary level platinum micro-coils (control group)as a control.The level of serum copper ions and the liver and renal function were recorded during different intervals before and after embolization.The arteriography and the tissue his-tology were observed respectively during different intervals after the embolization.Results 1 5 of 1 7 rabbits were embolized success-fully.After embolization,the level of serum copper ions increased in 2 weeks(P 0.05).The liver and renal function was similar to that of the preoperation after 2 weeks.After embolization,an-giography showed that vascular embolization effect between two groups was not significantly different at 10 min and 30 min;howev-er,the embolization effect of experimental group was superior to that of control one (P <0.01)at 3 days and 1 week,2 weeks,4 weeks,6 weeks and 12 weeks.Pathological results showed that there were a lot of thromboses inside,outside and around the copper coil.Few thromboses appeared around platinum coil in control group.The thrombosis situation in experiment group was better than that in control one (P <0.01).However,no significant difference in inflammatory cell infiltration between two groups was found. Conclusion Self-made secondary level copper plated platinum coil has good physical property,rememorability,flexibility and con-trollability.
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Objective:CD4+CD25+regulatory T cells (Treg) may contribute to tumor progression by suppressing antitumor im-munity. The function of Treg in antitumor immunity regulation in the peritoneal microenvironment of ovarian cancer (OC) was investi-gated and compared with the circulating Treg to elucidate OC immune escape. Methods: Flow cytometry was used to determine the proportion of CD4+CD25+T cells in CD4+T cells in ascites of 27 patients with OC and in peripheral blood lymphocytes of 28 patients with OC. The samples were analyzed and classified in three stages:primary disease (PD), after chemotherapy (AC), and recurrence dis-ease (RD), according to the clinical conditions of the OC patients upon donating the samples. The percentage of Treg in the three groups was determined in ascites and blood. CD4+CD25+T cells were isolated from ascites and peripheral blood of patients with OC us-ing magnetic sorting (MACS) system. The cells were then tested for regulatory function through coculture with carboxyfluorescein diac-etate succinimidyl ester-labeled autologous CD4+ CD25- responder cells. Results:The proportion of CD4+ CD25+T cells in CD4+T cells significantly increased in ascites (28.25%± 14.06%) compared with that in blood (14.6%± 4.74%;P<0.0001). The Treg in ascites and blood in AC showed higher proportion (P<0.0001) than those in the PD and RD;the proportion in RD was higher than that in PD (P<0.0001). Moreover, the Treg in ascites mediated a significantly higher suppression compared with the Treg in peripheral blood (P<0.001). Conclusion:The frequency and suppressor function of Treg were significantly higher in ascites than in peripheral blood. This finding suggests more possibility for escape immune surveillance in the peritoneal microenvironment. Moreover, the proportion of Treg in AC was higher than that in PD or RD;the proportion in RD was higher than that in the PD. Chemotherapy may favor the expansion of Treg, which may promote the recurrence of cancer.
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Objective: This research explores the relationship between the immuno-suppression function of regulatory T cells (Treg) in the ascites of ovarian cancer (OC) patients, the clinico-pathologic features of these patients, and the correlation of the function of Treg with initial treatment and relapse status of the patients to further investigate the specific mechanism of immuno-regulatory func-tion of CD4+ CD25+ Treg in the ascites of OC. Methods: Immuno-magnetic activated cell sorting (MACS) was conducted to sort CD4+CD25+Treg and autologous CD4+CD25-Treg from the ascites of 28 OC patients. Carboxyfluorescein-diacetate succinimidyl ester (CFSE) was used to label the autologous CD4+CD25-Treg. These labeled cells were then used as controls and co-cultured with autologous CD4+CD25+Treg at the ratio of 1∶1 or 1∶2. The mean inhibition ratio of Treg in specimens to the proliferation of autolo-gous CD4+ CD25-Treg was calculated after the flow cytometry of the CFSE expression and Modfit software analysis of the CD4+CD25-Treg proliferation index (PI) were performed. Anti-IL-10 and/or anti-TGF-β1 antibodies were neutralized to investigate whether the CD4+CD25+Treg-mediated immuno-suppression escaped through the ascites can produce a marked effect by the inhibitory cyto-kine IL-10 or TGF-β1. Results: The mean inhibition ratio of CD4+ CD25- Treg in the ascites of stage Ⅲ to Ⅳ OC patients was (75.72±17.04)%, which is significantly higher than that of stageⅠtoⅡOC patients (59.61±16.97)%;P<0.05. In addition, Treg in the as-cites of OC patients with recurrent disease showed a significantly higher inhibition ratio than that of patients with primary disease;P<0.001. Moreover, Treg in groups added into neutralizing anti-IL-10 and/or anti-TGF-β1 antibodies displayed significantly lower depres-sant effect than the control group;P<0.05. Conclusion:The immuno-suppression of CD4+CD25+Treg in the ascites of OC patients is correlated with the tumor staging and status of the primary or recurrent diseases. Moreover, Treg may indicate a suppressor function by secreting cytokine IL-10 and TGF-β1.